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KMID : 0894520140180030127
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Development & Reproduction
2014 Volume.18 No. 3 p.127 ~ p.131
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Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17a-Methyltestosterone
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Lee Chi-Hoon
Hur Sang-Woo
Lee Young-Don
Na Oh-Soo
Baek Hae-Ja
Noh Choong-Hwan
Han Sang-Hyun
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Abstract
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We investigated the androgenic effects of 17a-methyltestosterone (MT) on gonadal sex reversal in juvenile red spotted grouper Epinephelus akaara. The fish were immersed in 17a-MT at 1 and 5 mg/L. Treatment method of 17a-MT was once weekly for 4 and 8 weeks. Fish were sampled at 12 months after end of the treatment period in order to histological analysis. At the initiation of an experiment (70 day after hatching), juvenile red spotted grouper have the paired primordial gonads with somatic cells bellow kidney in the posterior portion of the body cavity. Formation of ovarian cavity indicates that the ovarian differentiation beginning at 70 DAH in red spotted grouper. At 12 months after end of the treatment period, control group, 17a-MT 1 mg/L treatment group for 4 and 8 weeks, and 17a-MT 5 mg/L treatment group for 4 weeks were all female. However, sex-changed males without ovarian cavity were observed in the 17a-MT 5 mg/L treatment group for 8 weeks. In grouper, we firstly reported that the red spotted grouper be able to induce the primary males by hormone treatment prior to gonadal sex differentiation.
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KEYWORD
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Red spotted grouper, Epinephelus akaara, Gonadal sex differentiation, Sex change, Primary males
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